Toxicity of Pseudomonas aeruginosa exotoxin A for human macrophages

Purification and characterization of Pseudomonas aeruginosa exotoxin.

A trypsin-sensitive, heat-labile exotoxin of Pseudomonas aeruginosa strain P-A-103 has been purified by a procedure that includes membrane ultrafiltration, hydroxylapatite chromatography, ion-exchange cellulose chromatography, and gel filtration chromatography. The procedure resulted in the recovery of 48% of the exotoxin with a 40-fold increase in specific activity (micrograms of protein per m...

Enzyme-linked immunosorbent assay for Pseudomonas aeruginosa exotoxin A.

An enzyme-linked immunosorbent assay (ELISA) is described for Pseudomonas aeruginosa exotoxin A. A double antibody sandwich method was used, employing polyvinyl microtiter plates as the solid phase, a primary coat of monospecific rabbit antitoxin serum, an outer layer composed of a horseradish peroxidase-sheep antitoxin immunoglobulin G conjugate, and an ortho-phenylene-diamine substrate. Absor...

Pseudomonas aeruginosa exotoxin: effect on cell cultures.

Induction of interleukin-1 from murine peritoneal macrophages by Pseudomonas aeruginosa exotoxin A.

Pseudomonas exotoxin A, an ADP-ribosylating toxin produced by Pseudomonas aeruginosa, has been shown to stimulate the proliferation of murine thymocytes, which requires the participation of accessory cells. This requirement for accessory cells can be replaced by supernatant from adherent peritoneal exudate cells that have been stimulated with exotoxin A. Antibody to exotoxin A inhibits the indu...

PREPARATION AND EVALUATION OF PROPERTIES OF PSEUDOMONAS AERUGINOSA EXOTOXIN A CONJUGATE WITH GOLD NANOPARTICLES

Background & Aims: Pseudomonas aeruginosa is an opportunistic pathogen and most important cause of hospital infections, which causes septicemia and death through various virulence factors such as exotoxin A. In the present study, P. aeruginosa exotoxin A (as a toxic factor) was prepared with the aim of obtaining a new recombinant vaccine candidate and conjugated with gold nanoparticles and its ...